Analysis of Osteocalcin (Human) by ELISA (CAT#: STEM-MB-1105-LGZ)

Introduction

Osteocalcin (OC), also known as R-hydroxy glutamic acid protein (GLa protein), is a kind of protein composed of 49 amino acids. It belongs to the non-collagen acid glycoprotein, which is a vitamin K dependent calcium binding protein. It is mainly synthesized by osteoblasts, odontoblast cells, and some by proliferative chondrocytes. It plays an important role in regulating bone calcium metabolism, and is a new biochemical marker for the study of bone metabolism, which has important value for the diagnosis of osteoporosis syndrome and abnormal calcium metabolism and other diseases.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Bone Disorders

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma

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