Analysis of PIGC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0575-LGZ)

Introduction

Official Full Name: phosphatidylinositol glycan anchor biosynthesis class C
Also known as: GPI2; MRT62; GPIBD16
This gene encodes an endoplasmic reticulum-associated protein involved in the biosynthesis of glycosylphosphatidylinositol (GPI) lipid anchors. The GPI lipid anchor is a glycolipid found in many blood cells and is used to anchor proteins to the cell surface. The encoded protein is a subunit of GPI n-acetylglucosamine (GlcNAc) transferase, which transfers GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. For this gene, two alternatively spliced transcripts encoding the same protein have been found. A pseudogene on chromosome 11 has also been identified.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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