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Analysis of PMS2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1853-LGZ)

Introduction

Official Full Name: PMS1 homolog 2, mismatch repair system component<br />Also known as: MLH4; PMS-2; PMSL2; HNPCC4; LYNCH4; MMRCS4; PMS2CL<br />The protein encoded by this gene is a key component of the mismatch repair system, which functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms a heterodimer with the gene product of mutL homolog 1 (MLH1), forming a mutL-alpha heterodimer. The mutl-α heterodimer has endolytic activity, is activated after recognition of mismatches and insertion/deletion loops by muts-α and muts-β heterodimers, and is required for removal of mismatched DNA. The DQHA(X)2E(X)4E motif, which forms part of the nuclease active site, is found at the C-terminus of the protein encoded by this gene. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements