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Analysis of Polymyxin B in Bacterial Growth Media by Liquid Chromatography–Mass Spectrometry (LC–MS) (CAT#: STEM-CT-1798-CJ)

Introduction

Polymyxin B is an antibiotic used to treat meningitis, pneumonia, sepsis, and urinary tract infections. While it is useful for many Gram negative infections, it is not useful for Gram positive infections. It can be given by injection into a vein, muscle, or cerebrospinal fluid or inhaled.




Principle

Liquid chromatography (LC) is a technique widely used to separate compounds from a sample prior to analysis and is frequently coupled to mass spectrometry (MS). With LC, separation of the sample components is based on the interactions of the compounds with the mobile and stationary phases, and the degree of compound separation is related to each compound's affinity for the mobile phase. Following chromatographic separations, compounds elute off of the column, desolvated into the gas phase and ionized at an ionization source, and are then introduced into the mass spectrometer for mass analysis.

Applications

Biochemistry; Biomedical; Biopharmaceuticals

Procedure

1. Sample Collection
2. Sample Preparation
3. Standardization and Calibration
4. Chromatographic Separation and Analysis

Materials

• Sample: Blood; Urine; Plasma; Crops; Livestock tissues; Milk; Eggs; Soil; Water; Sediment; Nucleotides; Peptides; Steroids; Hormones; Dyes; Fatty acids; Alcohols
• Equipment: Liquid-phase separation system; Mass spectrometer; Liquid chromatograph mass spectrometers
• LC-MS grade water; Acetonitrile
• Ion pairing agents: Trifluoroacetic acid (TFA); Formic acid (FA); Heptafluorobuteric acid (HFBA)

Notes

1. LC-MS is a dominant analytical technique that has a very high accuracy, sensitivity, and specificity.
2. Although known for its key features in providing rapid analysis of complex mixtures and quality performance, LC-MS is an expensive instrument.