Analysis of PRAMEF6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1391-LGZ)

Introduction

Official Full Name: PRAME family member 6
Enables ubiquitin ligase-substrate adaptor activity. Involved in proteasome-mediated ubiquitin-dependent protein catabolic process. Part of Cul2-RING ubiquitin ligase complex.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Clostridium Perfringens Types B by Real-Time PCR Method (CAT#: STEM-MB-2637-LGZ)
Analysis of Aethina Tumida by Real-Time PCR Method (CAT#: STEM-MB-2939-LGZ)
Analysis of Prosthogonimus Macrorchis by Real-Time PCR Method (CAT#: STEM-MB-4054-LGZ)
Analysis of CDC42EP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1814-LGZ)
Analysis of OR51E1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2332-LGZ)
Analysis of NGEF Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1760-LGZ)
Analysis of YOD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0292-LGZ)
Analysis of Streptococcus Mutans by Real-Time PCR Method (CAT#: STEM-MB-3136-LGZ)