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Analysis of PRDM12 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1946-LGZ)

Introduction

Official Full Name: PR/SET domain 12
Also known as: PFM9; HSAN8
This gene encodes a transcriptional regulator of sensory neuron specification, which plays a key role in pain perception. The encoded protein contains an n-terminal PRDI-BF1 and RIZ homology (PR) domain, a SET domain and three c-terminal C2H2 zinc finger DNA-binding domains. Natural mutations in this gene are associated with congenital pain insensitivity (CIP) and hereditary sensory and autonomic neuropathy (HSAN), which affects peripheral sensory and autonomic neurons. Deletion of this gene is associated with solid cancers and hematological malignancies including chronic myelogenous leukemia.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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