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Analysis of PRKCE Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1757-LGZ)

Introduction

Official Full Name: protein kinase C epsilon<br />Also known as: PKCE; nPKC-epsilon<br />Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that are activated by calcium and the second messenger diacylglycerol. Members of the PKC family phosphorylate a variety of protein targets and participate in a variety of cellular signaling pathways. PKC family members are also major receptors for a class of tumor promoter phobol esters. Each member of the PKC family has a specific expression profile and is thought to play distinct roles in the cell. The protein encoded by this gene is a member of the PKC family. This kinase has been shown to be involved in many different cellular functions such as neuronal channel activation, apoptosis, myocardial ischemic protection, heat shock response, and insulin exocytosis. Mouse knockout studies suggest that this kinase is important for activating lipopolysaccharide (LPS)-mediated signaling in macrophages and may also play a role in the control of anxiety-like behaviors.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements