Analysis of PRPF3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0586-LGZ)

Introduction

Official Full Name: pre-mRNA processing factor 3
Also known as: PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90
Intron removal from nuclear pre-mRNAs occurs at the spliceosome, which consists of four small nuclear ribonucleoprotein (snRNP) particles and an unknown number of transiently associated splicing factors. This gene product is one of several proteins associated with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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