Analysis of Pseudorabies Virus (PrV) by Real-Time PCR Method (CAT#: STEM-MB-2768-LGZ)

Pseudorabies Virus (PrV) causes Pseudorabies, and the clinical symptoms and course of disease of infected pigs vary greatly with age: Suckling piglets were the most sensitive, and piglets under 15 days of age often showed the most acute type, which was mainly manifested as high body temperature, diarrhea, shivering, uncoordinated movement, salivation, stiff neck muscles, watery movements of limbs, and finally coma and death. Most of the fattening pigs were associated with elevated body temperature, dyspnea, and generally did not die. After tolerance, the pigs showed a long latent infection with poison or detoxification. Adult pigs often show no visible clinical symptoms or only mild hyperthermia, and generally do not die. In the early gestation of sows, miscarriage may occur about 20 days after infection. In the late gestation, stillbirth and mummification, or weak and stillbirth, often occur. Pseudorabies is considered to be the swine virus disease with the greatest economic impact in the areas where the purification of swine fever has been completed. Therefore, rapid and accurate identification of pseudorabies virus plays an important role in the prevention and quarantine of the disease.
Primers and probes in this experiment have been optimized with high sensitivity. Provide positive controls to distinguish false negative samples. The primers are designed based on pseudorabies virus and do not cross-react with the DNA of other virus strains.

It is only suitable for scientific research, not for clinical diagnosis.

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

• Sample Type: serum