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Analysis of PSP-D (Human) by ELISA (CAT#: STEM-MB-1146-LGZ)

Introduction

PSP-D pulmonary surfactant protein, whose gene is located on the long arm of chromosome 10, is a macromolecular hydrophilic glycoprotein and belongs to the large family of calcium-dependent C-type lectins. PSP-D is composed of multimers, and there are different multimer forms among different species and different individuals. It plays an important role in maintaining the normal structure and function of the pulmonary surfactant phospholipids in the alveolar cavity, and in regulating local immunity and inflammation.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Neurobiology/Neurodegeneration

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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