Analysis of PTGDS Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2004-LGZ)

Introduction

Official Full Name: prostaglandin D2 synthase
Also known as: PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
The protein encoded by this gene is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of prostaglandin H2 (PGH2) to postprostaglandin D2 (PGD2). PGD2 functions as a neuromodulator and trophic factor in the central nervous system. PGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. This gene is preferentially expressed in the brain. Studies of transgenic mice that overexpress the gene suggest that the gene may also be involved in the regulation of non-REM sleep.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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