Analysis of PTPN22 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1145-LGZ)

Introduction

Official Full Name: protein tyrosine phosphatase non-receptor type 22
Also known as: LYP; PEP; LYP1; LYP2; PTPN8; PTPN22.5; PTPN22.6
This gene encodes a non-receptor class 4 subfamily member of the protein tyrosine phosphatase family. The encoded protein is a lymphocyte-specific intracellular phosphatase associated with the molecular adapter protein CBL, and may be involved in regulating the function of CBL in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune diseases, including type 1 diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternately spliced transcript variants encoding different isoforms have been described.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PTCH1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1937-LGZ)
Analysis of Norwalk Viruses (NV) by Real-Time PCR Method (CAT#: STEM-MB-3244-LGZ)
Analysis of Zika Virus (ZIKV) by Real-Time PCR Method (CAT#: STEM-MB-3510-LGZ)
Analysis of Chicken Infectious Anemia Virus (CIAV) by Real-Time PCR Method (CAT#: STEM-MB-2671-LGZ)
Analysis of Human Rhinovirus 9 by Real-Time PCR Method (CAT#: STEM-MB-3269-LGZ)
Analysis of DNAJC11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1293-LGZ)
Analysis of TMEM63A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0728-LGZ)
Analysis of TSC1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1944-LGZ)