Analysis of RHOQ Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1756-LGZ)

Introduction

Official Full Name: ras homolog family member Q
Also known as: ARHQ; TC10; TC10A; RASL7A; HEL-S-42
This gene encodes a member of the Rho family of small GTPases that cycles between inactive and active GTP-bound states and acts as a molecular switch in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton, regulating cell shape, attachment and motility. The encoded protein is an important signal protein for leiomyoma assembly, which has been shown to play an important role in the extracellular secretion of solute carrier family 2, glucose transporter member 4 and other proteins, and may be a signal to open the membrane fusion mechanism. Three associated pseudogenes were identified on chromosomes 2 and 14.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of NUF2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0508-LGZ)
Analysis of Yellow Fever Virus (YFV) by Real-Time PCR Method (CAT#: STEM-MB-3039-LGZ)
Analysis of WNT3A Gene (Mutation) in Hereditary Spastic Paraplegia by RT-qPCR (CAT#: STEM-MT-0203-LGZ)
Analysis of COX7A2L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1716-LGZ)
Analysis of RGS13 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0629-LGZ)
Analysis of CCN3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1905-LGZ)
Analysis of BIRC6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1795-LGZ)
Analysis of Infectious Bovine Rhinotracheitis Virus (IBRV) by Real-Time PCR Method (CAT#: STEM-MB-3878-LGZ)