Analysis of RIT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0615-LGZ)

Introduction

Official Full Name: Ras like without CAAX 1
Also known as: NS8; RIT; RIBB; ROC1
This gene encodes a member of a subfamily of Ras-related GTPases. The encoded protein is involved in regulating p38 MAPK-dependent signaling cascades related to cellular stress. This protein also cooperates with nerve growth factor to promote neuronal development and regeneration. Alternate splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of CRCT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0878-LGZ)
Analysis of Lsospora Suis by Real-Time PCR Method (CAT#: STEM-MB-3741-LGZ)
Analysis of SULT1C4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1452-LGZ)
Analysis of UICLM Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1661-LGZ)
Analysis of FH Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0975-LGZ)
Analysis of Radix Saposhnikoviae by Real-Time PCR Method (CAT#: STEM-MB-3981-LGZ)
Analysis of AIV-H5N1 Ag by Real-Time PCR Method (CAT#: STEM-MB-2907-LGZ)
Analysis of PRAMEF8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1389-LGZ)