Analysis of S100A4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0330-LGZ)

Introduction

Official Full Name: S100 calcium binding protein A4
Also known as: 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
The protein encoded by this gene is a member of the S100 protein family, which contains two EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a variety of cells and are involved in the regulation of many cellular processes, such as cell cycle progression and differentiation. The S100 gene includes at least 13 members, which are located on chromosome 1q21. This protein may play a role in motility, invasion and tubulin polymerization. Chromosomal rearrangements and expression changes of this gene are associated with tumor metastasis. Multiple alternative splice variants encoding the same protein have been identified.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PDC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0558-LGZ)
Analysis of LBH Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1813-LGZ)
Analysis of TSPAN31 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2500-LGZ)
Analysis of Equine Herpesvirus 2 (EHV-2) by Real-Time PCR Method (CAT#: STEM-MB-3172-LGZ)
Analysis of Fructus Liquidambaris by Real-Time PCR Method (CAT#: STEM-MB-3954-LGZ)
Analysis of Caprine Herpesvirus (CpHV) by Real-Time PCR Method (CAT#: STEM-MB-2680-LGZ)
Analysis of IRF7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2259-LGZ)
Analysis of Fructus Trichosanthis by Real-Time PCR Method (CAT#: STEM-MB-3979-LGZ)