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Analysis of S100B (Human) by ELISA (CAT#: STEM-MB-1164-LGZ)

Introduction

S100B protein (S100B), also called S100 calcium-binding protein B, is a protein of the S-100 protein family, located on chromosome 21q22.3. It is glial-specific and is expressed primarily by but not all astrocytes, it is only expressed by the subtype of mature astrocytes that wrap the sheath vessels and by cells expressing NG2. This protein may play a role in neurite extension, melanoma cell proliferation, stimulation of Ca2+ flux, PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Neurobiology/Neurodegeneration

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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