Analysis of Semliki Forest Virus (SFV) by Real-Time PCR Method (CAT#: STEM-MB-3887-LGZ)

Introduction

Semliki Forest virus (SFV) is an RNA virus in the Togaviridae family, Alphavirus genus. It was first isolated from mosquitoes in the Semliki Frost in Uganda in 1942. It causes infection and illness in humans and animals, including wild birds, rodents, domestic animals, and nonhuman primates.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.