Analysis of SIGMAR1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1942-LGZ)

Introduction

Official Full Name: sigma non-opioid intracellular receptor 1
Also known as: SRBP; ALS16; DSMA2; OPRS1; SR-BP; SIG-1R; SR-BP1; sigma1R; hSigmaR1
The gene encodes a receptor protein that interacts with a variety of psychomimetic drugs, including cocaine and amphetamines. This receptor is thought to play an important role in the cellular functions of various tissues related to the endocrine, immune and nervous systems. Like its previous name, Opioid Receptor Sigma 1 (OPRS1), the product of this gene was mistakenly identified as an opioid receptor; it is now thought to be a non-opioid receptor. Mutations in this gene have been linked to juvenile amyotrophic lateral sclerosis. Alternative splicing of this gene results in transcript variants encoding different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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