Analysis of SKI Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1404-LGZ)

Introduction

Official Full Name: SKI proto-oncogene<br />Also known as: SGS; SKV<br />This gene encodes the nuclear protooncogene protein homolog of avian sarcoma viral (v-ski) oncogene. It functions as a repressor of TGF-beta signaling, and may play a role in neural tube development and muscle differentiation.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Hypr Virus by Real-Time PCR Method (CAT#: STEM-MB-3166-LGZ)
Analysis of PRAMEF19 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1378-LGZ)
Analysis of Clostridium Sporogenes by Real-Time PCR Method (CAT#: STEM-MB-2634-LGZ)
Analysis of TRIM46 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0285-LGZ)
Analysis of PDE4DIP Gene (Mutation) in Hepatocellular Carcinoma by RT-qPCR (CAT#: STEM-MT-0138-LGZ)
Analysis of KCNJ13 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1538-LGZ)
Analysis of SPRR2G Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0687-LGZ)
Analysis of Shewanella Putrefaciens by Real-Time PCR Method (CAT#: STEM-MB-3064-LGZ)