Analysis of skin lipid conditions by Electron paramagnetic resonance (EPR) spectroscopy (CAT#: STEM-MB-1045-WXH)

Introduction

Lipids are the skin's natural fats. There are 2 kinds of lipids: epidermal and sebaceous. - Epidermal lipids are a mix composed of ceramides, free fatty acids and cholesterol. - Sebaceous lipids include triglycerides, wax esters, and squalene. The epidermal lipids of keratinocyte origin play an essential role in the skin's barrier function. These lipids provide a barrier against the movement of water and electrolytes as well as a barrier against microorganism invasion.

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Principle Applications Procedure Materials Notes Related Products

Principle

Electron Paramagnetic Resonance (EPR), also called Electron Spin Resonance (ESR), is a branch of magnetic resonance spectroscopy which utilizes microwave radiation to probe species with unpaired electrons, such as radicals, radical cations, and triplets in the presence of an externally applied static magnetic field.
EPR spectroscopy is particularly suitable for the investigation of (bio)chemical systems with strongly localized spin density and their interaction with the environment. For these systems EPR provides information on the structure and dynamics and is widely used in chemistry, physics and biology.

Applications

• Study dynamic organisation of lipids in biological membranes, lipid-protein interactions and temperature of transition of gel to liquid crystalline phases.
• Determine oxygen levels in tissues and blood.
• Injection of spin-labeled molecules allows for electron resonance imaging of living organisms.
• EPR can be used to measure microviscosity and micropolarity within drug delivery systems as well as the characterization of colloidal drug carriers.
• The study of radiation-induced free radicals in biological substances (for cancer research).
• Investigation on the antioxidant properties of medicine

Procedure

1. Sample Preparation
2. Electron paramagnetic resonance (EPR) spectroscopy testing
9. Data analysis

Materials

• EPR Spectrometer
• Spectrophotometer

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