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Analysis of SMG7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0311-LGZ)

Introduction

Official Full Name: SMG7 nonsense mediated mRNA decay factor<br />Also known as: EST1C; SGA56M; C1orf16<br />This gene encodes a protein that is critical for nonsense-mediated mRNA decay (NMD); a process in which transcripts with premature stop codons are rapidly degraded by the mRNA decay complex. The mRNA decay complex is partly composed of this protein together with the proteins SMG5 and UPF1. The N-terminal domain of the protein is thought to mediate its association with SMG5 or UPF1, while the C-terminal domain interacts with the mRNA decay complex. Thus, this protein likely couples changes in the phosphorylation state of UPF1 to the degradation of NMD candidate transcripts. Alternative splicing results in multiple transcript variants encoding different isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements