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Analysis of SPRR2E Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0689-LGZ)

Introduction

Official Full Name: small proline rich protein 2E
This gene encodes members of a small family of proline-rich proteins that cluster on the epidermal differentiation complex on chromosome 1q21. The encoded protein, along with other family members, is an integral part of the keratinocyte envelope formed under the plasma membrane of terminally differentiated stratified squamous epithelium. This envelope acts as a barrier to extracellular and environmental factors. Seven SPRR2 genes (A-G) appear to have been homogenized by gene transformation, while others show greater differences in protein structure.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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