Analysis of St.Louis Encephalitis Virus (SLEV) by Real-Time PCR Method (CAT#: STEM-MB-3838-LGZ)

Introduction

St. Louis encephalitis virus (SLEV) is a virus spread by mosquitoes that can make people sick. Most people infected with SLEV don't have symptoms, but in rare cases, SLEV can cause swelling or inflammation of the brain (encephalitis) and lead to death. Older adults are the most likely to get very sick if they are infected.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.