Analysis of Swine Vesicular Disease Virus (SVDV) by Real-Time PCR Method (CAT#: STEM-MB-3523-LGZ)

Introduction

Swine vesicular disease (SVD) is a viral disease of pigs that is characterized by the formation of vesicles and erosions on the hooves and around the mouth. The clinical signs can vary in severity, but the illness is short and not life-threatening.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.