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Analysis of TARBP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0305-LGZ)

Introduction

Official Full Name: TAR (HIV-1) RNA binding protein 1<br />Also known as: TRM3; TRMT3; TRP185; TRP-185<br />HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces chromosomally integrated DNA during a replicative cycle. The transcriptional activator Tat activates HIV-1 gene expression depending on the RNA regulatory element (TAR) downstream of the transcription initiation site. This element forms a stable stem-loop structure that can bind the protein encoded by the gene or RNA polymerase II. This protein likely disengages RNA polymerase II from the TAR during transcription elongation. An alternatively spliced transcript of this gene may exist, but its full-length nature has not been determined.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements