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Analysis of TIMP-2 / CSC-21K by ELISA (CAT#: STEM-MB-1555-LGZ)

Introduction

Tissue metalloproteinase inhibitor 2 (TIMP2) gene is a member of the TIMP gene family. Among the four known members of the TIMP family, TimP-2 has not only the common characteristics of all TIMPs, but also its own unique characteristics. The proteins encoded by this gene family are natural inhibitors of matrix metalloproteinases (MMP), a group of peptidases involved in the degradation of extracellular matrix. TIMP2 acts as both an MMP inhibitor and an activator. TIMP-1 inhibited the activity of MMP-7, MMP-9, MMP-1 and MMP-3 better than TIMP-2, while TIMP-2 inhibited the activity of MMP-2 more effectively than other TIMPs. In melanocytes, the expression of TIMP2 gene may be regulated by MITF. Recent studies have found that TIMP2 plays an important role in hippocampal function and cognitive function.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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