Analysis of TIMP-4 by ELISA (CAT#: STEM-MB-1545-LGZ)

Introduction

In humans, tissue metalloproteinase inhibitor 4 (TIMP-4) is a protein encoded by the TIMP4 gene in the tissue metalloproteinase inhibitor family. There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. Among the four known members of the TIMP family, TimP-4 has features common to all TIMPs and its own unique features. Unlike TIMP-2, TIMP-4 does not promote the activation of MMP-2 precursors through activated MMP-14(MT1-MMP). TIMP-4 mRNA is expressed at high levels in the heart and at low levels in the kidney, pancreas, colon and testis. TIMP-4 is expressed in breast cancer, cervical cancer, endometrial cancer, glioma and choriocarcinoma.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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