Analysis of TNFAIP6/TSG-6 (Human) by ELISA (CAT#: STEM-MB-1226-LGZ)

Introduction

Tumor necrosis factor alpha-inducible protein 6 (TNFAIP6), also known as TNF-stimulated gene 6 protein or TSG-6, is a protein encoded by the TNFaIP6 gene. It is a 30 kDa secreted protein containing a LINK domain a that binds hyaluronan and is thus a member of the hyaluronan-binding protein family, also known as hyaluronectin. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable covalent complex with inter-alpha inhibitor (IαI), thereby enhancing the serine protease inhibitory activity of IαI, which is important in protease networks associated with inflammation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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