Analysis of TNFSF12 / TWEAK / APO3L by ELISA (CAT#: STEM-MB-1567-LGZ)

Introduction

Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) was discovered in 1997. TWEAK the protein encoded by the tweak gene is a cytokine in the tumor necrosis factor (TNF) ligand family. White blood cells (including human resting and activated monocytes, dendritic cells and natural killer cells) are the main source of TWEAK. TWEAK induces apoptosis through a variety of pathways, depending on the cell type, and also promotes endothelial cell proliferation and migration, which can regulate angiogenesis. In chronic injury, over-activated TWEAK pathways can promote pathological tissue changes, including chronic inflammation, fibrosis, and angiogenesis.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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