Analysis of TNNT2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0175-LGZ)

Introduction

Also known as: CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6<br />This gene encodes the cardiac isoform of troponin T. The encoded protein is a tropomyosin-binding subunit of the troponin complex, located on the filaments of striated muscles, and regulates muscle contraction in response to changes in intracellular calcium ion concentrations. Mutations in this gene are associated with familial hypertrophic cardiomyopathy and dilated cardiomyopathy.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Cardiovascular phenotype, Hypertrophic cardiomyopathy 2, Dilated cardiomyopathy 1D, Cardiomyopathy, familial restrictive, 3, Cardiomyopathy, Primary familial hypertrophic cardiomyopathy, Hypertrophic cardiomyopathy

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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