Analysis of TRIM21 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2184-LGZ)

Introduction

Official Full Name: tripartite motif containing 21
Also known as: SSA; RO52; SSA1; RNF81; Ro/SSA
This gene encodes a member of a tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a polypeptide and one of four small RNA molecules. RoSSA particles exist both in the cytoplasm and in the nucleus. RoSSA interacts with autoantigens in patients with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing transcript variants of this gene have been described, but the full-length nature of only one has been determined.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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