Analysis of TRIM5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2180-LGZ)

Introduction

Official Full Name: tripartite motif containing 5
Also known as: RNF88; TRIM5alpha
The protein encoded by this gene is a member of the TRIM family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coil region. The protein forms homo-oligomers through the coil-coil region and localizes to the cytoplasm. It appears to act as an E3 ubiquitin ligase and ubiquitinates itself to regulate its subcellular localization. It may play a role in restricting retroviruses. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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