Analysis of Trypanosoma Brucei Brucei by Real-Time PCR Method (CAT#: STEM-MB-2945-LGZ)

Introduction

Trypanosoma brucei spp. (comprising Trypanosoma brucei brucei and the human infective forms T. b. rhodesiense and T. b. gambiense) are early branching, flagellated protozoan parasites causing sleeping sickness in human and nagana in cattle in 36 countries of sub-Saharan Africa, where they impose devastating impacts on human health and agriculture.
A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.