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Analysis of TWEAK/TNFSF12 (Human) by ELISA (CAT#: STEM-MB-1252-LGZ)

Introduction

Tumor necrosis factor ligand superfamily member 12 (TNFSF12), also known as TNF-related weak inducer of apoptosis (TWEAK), is a protein that is encoded by the TNFSF12 gene in humans. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF but displays a broader tissue distribution. Leukocytes are the main source of TWEAK, including human quiescent and activated monocytes, dendritic cells, and natural killer cells. TWEAK can induce apoptosis in a cell type-specific manner through multiple cell death pathways. This cytokine has also been found to promote the proliferation and migration of endothelial cells, thus acting as a regulator of angiogenesis.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or supernatant