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Analysis of UBE2J2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1436-LGZ)

Introduction

Official Full Name: ubiquitin conjugating enzyme E2 J2
Also known as: UBC6; NCUBE2; NCUBE-2; PRO2121
Ubiquitin modification of proteins is an important cellular mechanism for the degradation of abnormal or short-lived proteins. Ubiquitination involves at least three classes of enzymes: ubiquitin activating enzymes (E1s), ubiquitin conjugating enzymes (E2s) and ubiquitin protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located on the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some variants has not been determined.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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