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Analysis of ZP4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0272-LGZ)

Introduction

Official Full Name: zona pellucida glycoprotein 4<br />Also known as: ZBP; ZP1; ZPB; ZP1B; ZPB2; Zp-4<br />The zona pellucida is the extracellular matrix that surrounds oocytes and early embryos. It is mainly composed of three to four glycoproteins with different functions during fertilization and pre-implantation development. The nascent protein contains an N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site and a C-terminal transmembrane domain. Presumably, furin cleavage results in the release of the mature protein from the plasma membrane and its subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, the gene was known as ZP1 or ZPB, and was thought to have a similar function to mouse ZP1. However, a human gene with higher similarity and chromosomal identity to mouse Zp1 was assigned the symbol Zp1, and this gene was assigned the symbol ZP4.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements