Applied Biosystems 7900HT Real-Time PCR, Applied Biosystems (CAT#: STEM-PE-2109-LGZ)

The manufacturer of Applied Biosystems 7900HT Real-Time PCR is Applied Biosystems. Model is 7900HT. The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility, TaqMan® low-density arrays, fully automated robotic loading, and now optional fast real-time PCR capabilities.

Condition: used
Calibrated Dye: SYBR® Green I, ROX™, NED™, JOE™, TAMRA™, VIC™, FAM™, TET™
Capacity: Up to 84 x 384-well plates with optional automation accessory
Detection Method: SYBR, Primer-Probe Detection
Dimensions: 72 cm (W) x 84 cm (D) x 64 cm (H) (with drawer open)
High-throughput Compatibility: High-throughput Compatible, Multiplexing
Optics: Extended-life 488 nm argon-ion laser excitation source, No filters (CCD acts as spectrograph with continuous detection 500 -650 nm)
Passive Reference Dye: ROX (Separate Tube), No ROX, Other Passive Reference Dye, ROX (Pre-mixed)
Reaction Speed: Fast
Reaction Volume Range: 5-20 µL
Run Time: 52 minutes (384-well in fast mode), <2 hrs (384-well in standard mode)
Sample Ramp Rate: Fast mode: ± 3.0 °C⁄sec, Standard mode: ± 1.6 °C⁄sec
Temperature Accuracy: ±0.25 °C of display temperature
Temperature Range (Metric): 4-100 °C
Temperature Uniformity: ±0.5 °C
Thermal Cycling System: Peltier-Based System
Throughput: >5,000 real-time PCR rxns per day (384-well block and automation accessory), >10,000 endpoint PCR rxns per hour (384-well block and automation accessory)
Weight: 114 kg (250 lbs) with automation accessory, 82 kg (180 lbs) without automation accessory
Block Format: Interchangeable
Dynamic Range: Up to 9 logs of linear dynamic range
Sensitivity: Down to 10 copies

1. Rapid PCR can shorten the standard 96-well operation time to 35 minutes, or the 384-well operation time to 55 minutes.
2. Continuous wavelength detection from 500-660 nm, multiple fluorophores can be used in a single reaction.
3. Detect gene expression levels, detect and quantify pathogens, perform allelic identification (SNP genotyping), and score the presence of gene sequences.
4. Compatible with 96- or 384-well plates (including TaqMan® Low Density Arrays).
5. The Enterprise Edition software is optional and can be used to provide data analysis tools, support 21 CFR Part 11 guidelines, and provide data integrity and security.
6. Hands-free loading and unloading provides true walk-through automation, increasing laboratory productivity.
7. Validated assay development guidelines save time and money.

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