BeyoCool™ Cell Freezing Container, Beyotime (CAT#: STEM-L-1819-LKN)

BeyoCool™ Cell Freezing Container is manufactured by Beyotime. This product is also known as BeyoCool™ Programed Cooler and BeyoCool™ Programed Cryopreservation Container, which is through a programmed gradient cooling to freeze cells as perfectly as possible.
This product adopts the "cooling ring" made of precious metal alloy material with strong heat conductivity and high heat capacity and a fine heat transfer mechanism. It does not need the assistance of isopropanol or other liquids to ensure that it is placed in the BeyoCool™ cell cryopreservation box. The cooling rate of cell samples in the -80°C refrigerator is about -1°C/min.

This product is not only suitable for all kinds of ordinary cells, but also for the cryopreservation of various primary cells, stem cells and other relatively delicate cells. The cell survival rate after cryopreservation is very high.

Cryopreserved cells follow the "slow freezing" principle, which is mainly to prevent cells from forming ice crystals during the freezing process and causing damage to the cells. Usually, dimethyl sulfoxide (DMSO) is added to the cryopreservation solution to lower the freezing point of the water in the cells to prevent crystal formation. The usual "slow freezing" is to gradually lower the temperature. For example, cells are placed in a refrigerator at -20°C for 1-2 hours, then transferred to a refrigerator at -80°C overnight, and then transferred to a liquid nitrogen tank. A simpler and better option is to put it in a device that can gradually lower the temperature, such as a programmed cell cryostat box, and place it in a -80°C refrigerator, and it is best to ensure that the cooling rate is about -1°C/min.

Conventional cell cryopreservation boxes need to add isopropanol, and use isopropanol as the main carrier for heat storage and dissipation. After the heat is dissipated from the surface of the cryopreservation box or small gaps, the heat is quickly dissipated from the isopropanol through convection and conduction, and then the heat in the cell cryopreservation tube is transferred outward through the isopropanol, so that each cryopreservation tube is compared The temperature is gradually reduced at a consistent speed, reaching a cooling effect of -1°C/min. However, isopropanol is a toxic and harmful reagent and is easy to volatilize. It needs to be replaced after a certain number of uses. Long-term use will not only cause certain pollution to the environment, but may also have a certain toxic effect on experimenters.

The outer box of this product is a polymer material with high insulation, high heat insulation and low heat capacity. When the freezer box is placed in a refrigerator at -80°C, the heat in the box is slowly dissipated through the surface of the freezer box and small gaps. Outside the box. In the process of heat dissipation in the box, the "cooling ring" made of precious metal alloy material is given priority to dissipate due to its excellent heat conduction ability and higher heat capacity, that is, the temperature drops first. Then with the "cold gathering ring" as the center, the surrounding heat is gradually dissipated and is preferentially absorbed by the "cold gathering ring". During this process, the temperature of each cell cryopreservation tube will also slowly drop relatively uniformly and evenly, and finally reach the temperature in the ultra-low temperature refrigerator.

Packaging: 1 pcs.

Features:
Innovative Design: Radially symmetrical, insulated, and solid core design. The main body is made of composite polymer material aviation temperature-insulating resin, which will not freeze your hands under freezing conditions. The box cover can be easily opened, and the precious metal alloy "poly The "cold ring" replaces isopropanol, which often needs to be replaced, as a heat buffer and adjustment carrier.
Convenient to Use: Without the aid of isopropanol or other liquids and additives, directly put the cryotube into the cell cryopreservation box, and then put it in the -80°C low-temperature refrigerator.
No Pollution: no need to add or replace isopropanol, no need to replace any parts can be reused for a long time, and can maintain the original performance for a long time.
Stable Cooling Rate: the cooling rate is basically maintained at -1°C/min. The cooling rate of all holes is consistently high, which has very little effect on the surrounding temperature in the low-temperature refrigerator.

Notes:
Please use a 1-2ml cryotube to freeze cells.
Before putting it in the refrigerator, make sure that the "cooling ring" is at the bottom of the freezer box and close the lid tightly.
The inside of the freezer box tube hole and the outside of the freezer tube should be kept dry before putting it in the refrigerator to avoid adhesion between the two after freezing, and the presence of water droplets during the freezing process will affect the uniformity of the temperature drop in the freezer box. After putting it in the refrigerator, avoid being close to other objects, and make sure that there is a gap of at least 5-10 cm around.
This product is not affected by alcohol, isopropanol, disinfectant, etc., but try to avoid high temperature above 60°C and ultraviolet radiation.
This product is limited to scientific research by professionals, not for clinical diagnosis or treatment, not for food or medicine, and not for storage in ordinary residences.
For your safety and health, please wear lab coats and disposable gloves.