Bio-Rad iCycler MyIQ PCR / Thermal Cycler, Bio-Rad Laboratories (CAT#: STEM-PE-2080-LGZ)

The manufacturer of Bio-Rad iCycler MyIQ PCR / Thermal Cycler is Bio-Rad Laboratories. Model is iCycler MyIQ. The MyiQ Real-Time PCR Detection System provides an affordable solution for the detection of common fluorescent green dyes such as FAM and SYBR green i. The system interfaces directly with the iCycler thermal cycler and is able to provide superior features such as thermal gradient capability and peltier effect-driven performance.

Condition: used
Temperature Range: 4 - 100 °C
Temperature Uniformity: ± 0.4 °C
Heated Lid Temperature: up to 100 °C
Gradient Mode
Gradient Accuracy: ± 0.4 °C
Row Uniformity: ± 0.4 °C
Gradient Range: 40 - 100 °C
Sample Capacity: 96 Wells
Sample Size: 15 - 100 µL
Dimensions (W x D x H): 29.2 x 58.4 x 38.7 cm
Fluorescence Excitation Range: 475 - 495 nm
Fluorescence Detection Range: 515 - 545 nm
Dynamic Range: 9 orders of magnitude

1. Nucleic acid amplification and detection is one of the most valuable technologies in biological research today. Real-time detection of PCR products is achieved by adding a fluorescent chemical to the reaction that reports an increase in the amount of DNA as the proportion of fluorescent signal increases. Fluorescent chemistries used for this purpose include DNA-binding dyes and fluorescently labeled sequence-specific primers or probes.
2. Real-time fluorescent quantitative PCR technology can accurately and sensitively quantify the copy number of the starting template in a large dynamic range. Fluorescence amplification was monitored using a dedicated thermal cycler equipped with a fluorescence detection module. The MyiQ™ Single Color Real-Time PCR Detection System is built on the iCycler® thermal cycler to provide optimal PCR performance.
3. The MyiQ system uses a tungsten halogen lamp as the light source. The filter-based optical design allows the use of optimal wavelengths of light for excitation and emission, resulting in superior sensitivity and resolution between multiple fluorophores. The CCD detector captured images of all 96 wells simultaneously. This resulted in a comprehensive dataset illustrating the kinetic behavior of the data at each cycle.
4. Simultaneous acquisition of images ensures reliable comparison of data between wells. The MyiQ system can report data during PCR in real time for instant feedback on the success of the reaction. All of these features suggest that building MyiQ system hardware can improve reliability and flexibility.
5. MyiQ software has functions that make the software simple and practical. The software is designed for convenience - providing quick setup and analysis results. For ease of use, many functions are presented graphically. As your mouse rolls over the button, you can get hints to use. In addition, the online help manual is available at any time by pressing the F1 key. MyiQ software automatically analyzes the collected data at the click of a button and leaves room for additional optimization of the results based on your analysis preferences.

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