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Biointeraction Analysis of Immobilized Antibodies and Related Agents by Immunoaffinity Chromatography (CAT#: STEM-ACM-0110-CJ)

Introduction

Antibodies have been used for decades as agents for the selective binding of target analytes. One common application of antibodies has been their use in immunoaffinity chromatography (IAC) for the purification and isolation of biological compounds. Many IAC methods have been developed for the isolation of antibodies, hormones, peptides, enzymes, viruses and other biologically-relevant materials. The ability of an antibody to recognize a specific target and bind this target with high affinity has traditionally provided immunoaffinity methods with good selectivity and low limits of detection.




Principle

Immunoaffinity chromatography is a method for separating target antibodies or antigens from a heterogeneous solution. It is column-based, which means that the solution is flowed through a column and eluted at the other end. The column is pre-functionalized with the capture antibody or antigen. The target protein is adsorbed onto the resin-bound capture protein and is retained in the column while the remaining solution is eluted. The fraction containing the target protein is later eluted and purified.

Applications

Biochemical; Biomedical

Procedure

1. Preparation of Column: The column is loaded with solid support such as sepharose, agarose, cellulose etc.. Ligand is selected according to the desired isolate. Spacer arm is attached between the ligand and solid support.
2. Loading of Sample: Solution containing a mixture of substances is poured into the elution column and allowed to run at a controlled rate.
3. Elution of Ligand-Molecule Complex: Target substance is recovered by changing conditions to favor elution of the bound molecules.

Materials

• Sample: Plants; Natural Food; Protein; Drug; Pollutants; Blood; Saliva; Serum; Plasma; Antibodies; Viruses & More
• Equipment: Agarose; Silica gel; Aluminium oxide; Acrylate; Organic polymers; Wash Buffer
• (Optional): Ligand; Spacer arm; Column

Notes

1. Immunoaffinity chromatography is one of the most widely used affinity-derived procedures, and it permits the production of ligands when the needed ligand is unavailable.
2. In this method, the stationary phase consists of an antibody or an agent linked to antibodies. Due to the high specificity of antibodies, it is possible to isolate varied substances using this method.
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