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BL652A RIPA Lysis Buffer (Weak), Biosharp Life Science (CAT#: STEM-GLE-4531-LGZ)

Highlights

The main components of RIPA lysate (weak) are 50 mM TrisHCl (pH 7.4), 150 mM NaCl, 1% NP40, 0.25% sodium deoxycholate, and Sodium Orthovanadate, Sodium Fluoride, EDTA, etc.
This product needs to be used together with conventional protease inhibitors to achieve better extraction results; this product contains phosphatase inhibitors, which can be used to extract phosphorylated proteins.
The protein sample obtained by lysing with RIPA lysis solution (weak) can be used to measure the protein concentration with the BCA protein concentration assay kit. Due to the high concentration of detergent, the Bradford method cannot be used to determine the protein concentration of the samples lysed by this lysate.

Cat Number: STEM-GLE-4531-LGZ

Application: For cell lysis.

Model: BL652A

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Description

RIPA lysate is a classic fast lysate of cell tissues, which has a strong lysing effect on animal cell membrane, cytoplasm, and nucleus components. The protein samples obtained from lysis can be used for conventional Western, IP, etc. The original meaning of RIPA is Radio Immunoprecipitation Assay. There are many formulations of RIPA lysate, which can be roughly divided into three categories: strong, medium and weak according to the strength of the lysate.

Specification

Specification: 100ml

Features

The main components of RIPA lysate (weak) are 50 mM TrisHCl (pH 7.4), 150 mM NaCl, 1% NP40, 0.25% sodium deoxycholate, and Sodium Orthovanadate, Sodium Fluoride, EDTA, etc.
This product needs to be used together with conventional protease inhibitors to achieve better extraction results; this product contains phosphatase inhibitors, which can be used to extract phosphorylated proteins.
The protein sample obtained by lysing with RIPA lysis solution (weak) can be used to measure the protein concentration with the BCA protein concentration assay kit. Due to the high concentration of detergent, the Bradford method cannot be used to determine the protein concentration of the samples lysed by this lysate.

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