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CaptureSelect™ IgG-Fc (ms) PhyTip® Columns (200 µL Volume) Containing 20 µL Affinity Media Per Column, PTT-92-20-24, Tecan (CAT#: STEM-C-4176-LGZ)

Highlights

PhyNexus, now part of Biotage, developed PhyTip® columns in 2002 and has supported researchers in automated protein purification since then. PhyTip columns purify samples through repeated back and forth cycles, a process known as dual-flow chromatography that allows for complete binding interactions during each purification step.

IgGs recovery 70 - 90%

Cat Number: STEM-C-4176-LGZ

Application: For purification of IgGs in various species.

Model: PTT-92-20-24

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Description

CaptureSelect™ IgG-Fc (ms) purifies IgG from various species by binding to the Fc region of IgG antibodies. This affinity medium is designed for the purification of IgGs from a variety of species, including mouse, cow, rabbit, rat, goat, horse, human and sheep. CaptureSelect™ IgG-Fc (ms) binds to a highly conserved epitope in the Fc region of the heavy chain. Unlike protein G, which is known to present challenges resulting in low yields, dissociated homodimers, and denatured antibodies under standard elution conditions, this product can be used under mild elution conditions to preserve the structure of the purified protein and functional integrity.

Affinity ligands for ThermoFisher's CaptureSelect™ media are based on recombinant antibody fragments of only 14 kDa in size and can be adapted for highly selective binding to certain moieties or antibodies and antibody fragments.

Specification

Pack Size: 96

Features

PhyNexus, now part of Biotage, developed PhyTip® columns in 2002 and has supported researchers in automated protein purification since then. PhyTip columns purify samples through repeated back and forth cycles, a process known as dual-flow chromatography that allows for complete binding interactions during each purification step.

IgGs recovery 70 - 90%
Less protein loss during purification due to maximized sample binding. The result is very high sample recovery.

Sample concentration and purification in one
Our unique packed resin bed column design results in the entire sample being bound to a small resin bed volume. Purified samples can be eluted in small volumes, resulting in highly concentrated samples.

IgG purification for 15 minutes
Efficient purification via PhyTip columns allows for a complete purification protocol of 96 samples in as little as 15 minutes.

Compatible with all major liquid handling systems
Our PhyTip columns are compatible with all major liquid handling platforms, enabling you to use your existing systems, which you may already have in your laboratory for automated protein purification. If you do not have a system, we can provide an automated solution for protein purification with PhyTip columns.

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