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Characterization of chitosan/ZnO nanoparticle composite membranes by UV-Vis Spectroscopy (CAT#: STEM-MB-0923-WXH)

Introduction

Chitosan (CS), a high molecular weight cationic polysaccharide, has attracted considerable interest because of its non-toxicity, good mechanical property, antibacterial activity, biocompatibility, and excellent film-forming ability. For this reason, CS has been applied in biomaterial, medicinal, environmental, and other industrial fields. Over recent years, hybrid materials based on CS have been developed, including conducting polymers, metal nanoparticles, and oxide agents, due to excellent properties of individual components and outstanding synergistic effects simultaneously.
Currently, the research on the combination of CS and metal oxide has focused on titanium dioxide, as titanium dioxide has excellent photocatalytic performance and is stable in acidic and alkaline solvents. Compared with titanium dioxide, zinc oxide has similar band-gap and antibacterial activity. It is well known that CS is only soluble in aqueous solutions of organic or mineral acids.




Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer
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