Characterization of Membrane Protein-Lipid Interactions by Ion Mobility Mass Spectrometry (CAT#: STEM-ST-0117-LJX)

Introduction

Lipids in the biological membrane can modulate the structure and function of integral and peripheral membrane proteins. Distinguishing individual lipids that bind selectively to membrane protein complexes from an ensemble of lipid-bound species remains a daunting task. Recently, ion mobility mass spectrometry (IM-MS) has proven to be invaluable for interrogating the interactions between protein and individual lipids, where the complex undergoes collision induced unfolding followed by quantification of the unfolding pathway to assess the effect of these interactions.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Ion mobility spectrometry–mass spectrometry (IMS-MS) is an analytical chemistry method that separates gas phase ions based on their interaction with a collision gas and their masses. In the first step, the ions are separated according to their mobility through a buffer gas on a millisecond timescale using an ion mobility spectrometer. The separated ions are then introduced into a mass analyzer in a second step where their mass-to-charge ratios can be determined on a microsecond timescale.

Applications

For studying the gas phase ion structure
For detecting the chemical warfare agents and explosives
For the analysis of proteins, peptides, drug-like molecules and nano particles
For monitoring isomeric reaction intermediates and probe their kinetics
For proteomics and pharmaceutical analysis

Procedure

1. Add sample
2. The ions in the sample are separated in the ion mobility spectrometer
3. The separated ions are introduced into the mass analyzer for detection
4. Store the detection results

Materials

• Sample Type:
Compound

Notes

1. Ion mobility spectrometry is also a very fast technique, making it suitable for high-throughput applications. The entire analysis can be completed in just a few minutes.
2. The method is extremely sensitive and able to detect trace amounts of contaminants that other spectrometry methods would miss.
3. The effective separation of analytes achieved with this method makes it widely applicable in the analysis of complex samples such as in proteomics and metabolomics.

Other recommended products

Structures of cobalt oxide cluster cations studied by ion mobility mass spectrometry (CAT#: STEM-ST-0175-LJX)
Separation of Peptide Isotopomers by Ion Mobility -Mass Spectrometry (CAT#: STEM-ST-0106-LJX)
Determination of drugs and drug metabolites by ion mobility-mass spectrometry (CAT#: STEM-ST-0086-LJX)
Protein analyses by differential ion mobility microchips with mass spectrometry (CAT#: STEM-ST-0105-LJX)
Conformational preferences of linear beta-defensins are revealed by ion mobility-mass spectrometry (CAT#: STEM-ST-0124-LJX)
Identification of carbohydrate anomers by ion mobility-mass spectrometry (CAT#: STEM-ST-0114-LJX)
Analysis of isomeric disaccharide precursor, product and cluster ions by ion mobility mass spectrometry (CAT#: STEM-ST-0160-LJX)
Both targeted and non-targeted analysis of phase II steroid metabolites in urine by ion mobility-mass spectrometry (CAT#: STEM-ST-0163-LJX)