ClinMax™ Human IFN-γ Kit (Flow Cytometry Beads Assay), ACROBiosystems (CAT#: STEM-CT-0674-ZJF)

This assay kit is used to measure the concentrations of Human Interferon-γ (IFN-γ) by employing a standard sandwich-FCM format. The antibody encapsulated 1-plex beads, protein (IFN-γ) in specimens and biotin-conjugated detection antibody formed a sandwich complex as bead-analyte-detection antibody. SA-PE was added in and react with biotinylated detection antibody, the intensity of PE fluorescence, in proportion to the recombinant protein titer in specimens, was assessed by flow cytometry at wavelength of 575 nm approximately. The intensity of APC fluorescence was applied to classify bead populations, at wavelength of 670 nm approximately.

Assay Type: Sandwich-FCM
Analyte: IFN-γ
Format: 96T
Reactivity: Human
Regulatory Status: RUO
Sensitivity: 2.0 pg/mL
Standard Curve Range: 9.8 pg/mL-10000 pg/mL
Assay Time: 2 hr 30 min
Suitable Sample Type: For the quantitative determination of human IFN-γ in Cell Culture Supernatants, Plasma, Serum.
Sample volume: 30 μL

Quick & Easy-to-use: 2 hour assay with only 7 steps required.
Consistent: Calibrated using NIBSC/WHO standards to ensure batch-to-batch consistency.
Strict Quality Control: High sensitivity, specificity, accuracy, linearity and inter/intra precision.
Validated Method: Comprehensive validation with available protocol and 24h professional technical support.
Minimal Matrix Interference: Low matrix interference verified in cell culture supernatant and serum.

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ClinMax™ Human Interferon-γ (IFN-γ) ELISA Kit, ACROBiosystems