The CytoSinct™ CD34 Nanobeads, human are used for separating CD34+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products or single cell suspension based on the surface expression of human CD34. The surface of Nanobeads are labeled with anti-human CD34 monoclonal antibody. To begin the isolation, Nanobeads are added to the cells. The CD34 antibodies preconjugated to the Nanobeads can bind the target cells expressing CD34 on cell surface. The cells/beads mixed suspension is loaded onto a CytoSinct™ Column which is effectively magnetized by an external magnetic field from CytoSinct™ Magnet, or other compatible cell isolation columns and magnets. The Nanobeads-labeled CD34+ cells are retained within the column and enriched during the wash step when Isolation Buffer is used to flush out the CD34- cells. After removing magnetic field, the target CD34+ cells can be easily eluted from the column.
Specification
Volume: 7.5 mL, Up to 4X10^10 total MNCs Reactivity: Human Format: Bio-degradable matrix coated nanoparticle conjugated with anti-CD34 antibodies supplied in phosphate buffered-saline (PBS), containing Human Serum Albumin (HSA), pH 7.0-7.4. Storage: Store at 2-8 °C. Do not freeze.
Features
Gmp Grade Ultra Purity High Recovery High Viability Nano-sized Bio-degradable Bio-compatible Cost Efficient