Detection of SARS-CoV-2 spike protein D614G mutation by RT-qPCR (CAT#: STEM-MT-0026-LGZ)

Introduction

Monitoring the spread of G614 in specific locations is critical because this variant is highly contagious and can trigger the emergence of other mutations. Therefore, a rapid, accurate and reliable method for detecting D614G mutations would be beneficial.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

SARS-CoV-2

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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