Determination of hybrid status in Lilium by Fluorescence in situ hybridisation (FISH) (CAT#: STEM-MB-1225-WXH)

Introduction

Wide hybridisation enables the introduction of desired genes, such as those encoding resistance to pathogens and adverse climatic conditions, to many plant crops and ornamentals. It is well known that not all plants obtained from wide crosses are hybrids. They may also arise apomictically from unfertilised maternal cells. This process may be induced by incompatible or sterile pollen. In the genus Lilium, the apomictic means of propagation was reported in Lilium regale, L. speciosum, L. canadense, L. szovitsianum, L. longiflorum, L. superbum and L. pumilum. Apomixis and the possibility of uncontrolled pollination make it necessary to confirm whether or not seedlings obtained from distant crosses are indeed desired hybrids.