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Determination of Water Content in Brazilian Honeybee-Collected Pollen by Karl Fischer Titration (CAT#: STEM-PPA-0129-YJL)

Introduction

Bee pollen has been a part of the human diet due to its high nutritional value and consists of approximately 40% carbohydrates, 35% proteins, 4–10% water, 5% lipids, and 5–15% other substances, such as amino acids, vitamins, minerals, antibiotics and antioxidant substances. It is popularly believed that regular consumption of bee pollen has a beneficial effect on several medical conditions, such as: depression, anemia, stress-related diseases, memory loss, intestinal and prostate problems, impotence, ageing, impaired immune functions, among others.
The water content of bee pollen is an important quality parameter or indicator, as it directly influences several basic characteristics of the product, in addition to directly affecting the product’s keeping quality and typical flavor, as well as product and aroma preservation. A high water content may increase the activity of microorganisms and enzymes, which in turn, may change sensory characteristics of the product. On the other hand, a much reduced water content may result in rapid rancidity.




Principle

Karl Fischer titration is used as a reference method for many substances and is a chemical analysis method based on the oxidation of sulfur dioxide by iodine in methanol hydroxide solution. Titration can be performed by volumetric or coulometric methods.
According to the volumetric method, an iodine-containing Karl Fischer solution is added until the first signs of iodine excess appear. Iodine turnover was determined by the volume of the burette containing iodine Karl Fischer solution.
In the coulometric procedure, reacted iodine is generated directly in the titration cell by electrochemical oxidation of iodide ions until traces of unreacted iodine are detected. Faraday's law can be used to calculate the amount of iodine produced by the required charge.

Applications

Food industry; Petroleum industry

Procedure

1. Pre-titrate the instrument.
2. Weigh the sample.
3. Pour the sample into the titration vessel.
4. Stir until the sample dissolves, and titrate to the end point with Karl Fischer titrant.
5. Enter the sample mass and record the moisture determination.

Materials

• Sample Type: Liquids, solids, slurries, even gases
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