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Plasma osmotic pressure is one of the important indicators to reflect the internal environment of the body. Healthy people have a perfect system to regulate the balance of osmotic pressure in the body, and all organs and tissues have almost the same osmotic pressure environment. Once the osmotic pressure balance of body fluids is disturbed or destroyed, it will cause the movement of water between various body fluids and between inside and outside cells, resulting in changes in the content of water, electrolyte and organic matter in body fluids, and finally lead to the disturbance of body function activities. Freezing point drop method is the "gold standard" of osmotic pressure.